DNA sequencing of microorganisms collected on habitat restoration trial plots in the Falkland Islands (2013-2014)

The dataset comprises of 8699324 DNA sequences (divided by phyla, class, order, family and genus) of microorganisms analysed from soil samples collected in the Falkland Islands. Soil samples came from habitat restoration plots at two different locations (Fitzroy ridge and Cape Pembroke). Aim of the data collection was to provide a first assessment of the biodiversity and functional potential of local soil microbial communities as the relationship between microbial diversity, soil types and plant cover are not well understood in the Falkland Islands. Geographic coordinates for each sampling point are associated to the dataset. Data has not been published yet in peer-reviewed journal (GenBank assess number to be confirmed)

Data and Resources

This dataset has no data

Additional Info

Field	Value
Last Updated	December 14, 2020, 09:54 (PST)
Created	January 30, 2020, 05:22 (PST)
Region	Falkland Islands
Language	eng
Topic Category	Biota; flora and/or fauna in natural environment
Temporal Extent Start	2013-01-01
Temporal Extent End	2014-12-31
Dataset Reference Date	2014
Lineage	The 16S rRNA gene taxon DNA sequences were obtained though Lab analyses undertaken at the Natural History Museum, Life Sciences Department (London, UK) in order to extract the taxon DNA sequences. Species diversity analyses were carried out to understand the composition of the bacteria and archaea in different types of soil. In parallel, similarities percentage at phyla level across unrestored and plot samples were calculated.DNA was extracted using PowerSoil (copyright) DNA Isolation Kit. 16S rRNA gene sequences were amplified by polymerase chain reaction (PCR) and sequenced using high-throughput sequencing on a MiSeq illumina platform. The sequence data was quality checked and processed using the sequencing processing as described by Qiime (http://qiime.org/) . The results of the analyses were published in the master thesis by Maria Balandina, september 2015. The thesis is available on request.
West Longitude	-58.4127
South Latitude	-51.921
East Longitude	-57.7619
North Latitude	-51.6904
Spatial Reference System	WGS84
Responsible Organisation Name	Hidden (personal data protection)
Contact Mail Address	Hidden (personal data protection)
Responsible Party Role	Hidden (personal data protection)
Access Limitations	Restricted until publication
Use Constraints	Open, but copyright and/or Intellectual Property Rights apply
Resource Reference	Cite data source as: Blandina M., Understanding the effect of habitat and soil type on microbial diversity of the Falkland Islands. (2015) Master thesis, Imperial College London.
Data Format	excel
Update Frequency	not planned
Accuracy	GPS position: between 1 and 5 metres
Resource Type	Dataset
Original Title	Hidden (internal use only)
Metadata Date	2017-03-27
Metadata Point of Contact	datamanager@saeri.ac.fk
Contact Consent	Contact details hidden
Unique Resource ID	FK-NHM-362

Dataset extent

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