Falkland Islands seaweed DNA barcode list
Data and Resources
This dataset has no data
Additional Info
| Field | Value |
|---|---|
| Last Updated | December 14, 2020, 09:53 (PST) |
| Created | January 30, 2020, 05:21 (PST) |
| Region | Falkland Islands |
| Language | eng |
| Topic Category | Biota; flora and/or fauna in natural environment |
| Temporal Extent Start | 2013-01-01 |
| Temporal Extent End | 2013-12-31 |
| Dataset Reference Date | 2013 |
| Lineage | DNA extractions were carried out using the DNeasy Plant Minit Kit (Qiagen, Hilden, Germany) on material from specimens preserved in silica gel which had been collected around the Falkland Islands. Polymerase chain reaction (PCR) was performed using a specific primer pair for the partial mitochondrial COI gene region (first fragment). PCR products were electrophoresed to check for amplification and correct length on a 1.2% (w/v) agarose gel prepared with Tris/Borate/EDTA buffer with post-staining in GelRed (Biotium). For sequencing a single reaction product of approximately 50 ng DNA was purified using the QIAquick PCR Purification Kit (Qiagen) and sequencing of both strands was carried out by the Source Bioscience sequencing service using the same primers as employed for PCR. Returned ABI files were imported into BioEdit for quality control and resulting consensus sequences were queried against the NCBI online database using BLASTn. Sequences have been deposited in the ENA/EBI online database and can be accessed through the list of the DNA barcodes. |
| West Longitude | -61.4548 |
| South Latitude | -52.9257 |
| East Longitude | -57.6865 |
| North Latitude | -50.9992 |
| Spatial Reference System | WGS84 |
| Responsible Organisation Name | Hidden (personal data protection) |
| Contact Mail Address | Hidden (personal data protection) |
| Responsible Party Role | Hidden (personal data protection) |
| Access Limitations | Open access |
| Use Constraints | Open, but copyright and/or Intellectual Property Rights apply |
| Resource Reference | Please acknowledge data source as: Alexandra Mystikou "Seaweed Biodiversity around the Antarctic Convergence in the South Atlantic" MSc September 2015, University of Aberdeen and South Atlantic Environmental Research Institute (SAERI) |
| Data Format | excel |
| Update Frequency | not planned |
| Accuracy | 100% confidence in the |
| Resource Type | Dataset |
| Original Title | Hidden (internal use only) |
| Metadata Date | 2016-02-10 |
| Metadata Point of Contact | datamanager@saeri.ac.fk |
| Contact Consent | Contact details hidden |
| Unique Resource ID | FK-SAERI-254 |
Dataset extent
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