MonthlyWaterQualitySampling_2021

A baseline study carried out by the Blue Belt programme at nine sites in April 2019 indicated that West Rocks Outfall and James Bay Outfall are at higher risk of contamination by faecal bacteria originating from terrestrial sewage sources. Four water samples were collected monthly from James and Ruperts Bay from September 2020 to provide data for a preliminary assessment of the extent of faecal contamination. CTD casts were also carried out to measure temperature and salinity at each of the four stations

Data and Resources

This dataset has no data

Additional Info

Field	Value
Last Updated	June 24, 2025, 05:48 (PDT)
Created	June 24, 2025, 05:48 (PDT)
Region	Saint Helena
Language	eng
Topic Category	Biota; flora and/or fauna in natural environment
Temporal Extent Start	2020
Temporal Extent End	2021
Dataset Reference Date	2021
Lineage	Sea water samples were collected at 4 stations from James bay to Ruperts bay (James bay: opposite the Jamestown run outflow, opposite the middles step entry point at the wharf, opposite the front step entry point at the wharf, Ruperts Bay: opposite the temporay slipway). Samples were collected in sterile glass bottles provided by the St Helena Public Health Laboratory at the General Hospital and placed in an icebox during collection and delived to the lab within 30 minute of fieldwork ending. Processing of water samples was caried out by the St Helena Public Health Laboratory at the General Hospital. The number of colony forming units of total coliforms and E. coli were enumerated using standard methods (SCA 2016) following ISO guidelines (ISO 2018). For each sample, up to 100 ml of water is filtered onto two 47 mm, 0.45 µm pore-size cellulose nitrate filter membranes. The filters are placed onto an absorbent pad that is saturated with membrane lauryl sulfate broth (MLSB) in a Petri dish. Petri dishes are incubated at 30 oC for a 4-h recovery step. Following recovery, one Petri dish is incubated at 37 oC and the other at 44 oC for at least 14 hours for each sample. After incubation, yellow colonies on the membranes incubated at 37 oC were counted as presumptive coliforms and yellow colonies on the membranes incubated at 44 oC were counted as presumptive E. coli. CTD casts were done along side water samples to give indcations of temperature and salinity at each site.
Spatial Reference System	EPSG4326
Responsible Organisation Name	Hidden (personal data protection)
Contact Mail Address	Hidden (personal data protection)
Responsible Party Role	Hidden (personal data protection)
Access Limitations	Restricted, data environmentally sensitive, however data is open to government departments
Use Constraints	Restricted, but open subject to limitations and prior agreement with responsible organisation. Copyright must be cited
Resource Reference	St Helena Government, Environmental Managament Division, Marine Conservation Section and Public Health
Data Format	pdf, CSV, Valeport data log
Update Frequency	Monthly
Accuracy	"PRT +/- 0.01ºC inductive cell +/- 0.01mS/cm strain gauge transducer +/- 0.05%"
Resource Type	DataSeries
Original Title	Hidden (internal use only)
Metadata Date	2021-06-30
Metadata Point of Contact	datamanager@saeri.ac.fk
Unique Resource ID	SH-SHG-289